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KMID : 0545120100200101386
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 10 p.1386 ~ p.1392
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Rheinheimera aquatica sp. nov., an Antimicrobial Activity Producing Bacterium Isolated from Freshwater Culture Pond
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Wen-Ming Chen
Chang-Yi Lin
Chiu-Chung Young
Shih-Yi Sheu
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Abstract
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A bacterial strain designated GR5T was isolated from freshwater culture pond in Taiwan during the screening of bacteria for antimicrobial compounds and characterized using the polyphasic taxonomic approach. Strain GR5T was Gram-negative, aerobic, greenish-yellow colored, rod-shaped, and motile by means of a polar single flagellum. Growth occurred at 10-40°C (optimum, 35°C), at pH 7.0-8.0 (optimum pH 8.0) and with 0-2.0% NaCl (optimum, 0.5-1.0%). The major fatty acids were C16:1ω7c (36.3%), C16:0 (16.6%), C12:0 3-OH (12.5%) and C18:1ω7c (9.1%). The major respiratory quinone was Q-8. The DNA G+C content of the genomic DNA was 51.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GR5T belonged to the genus Rheinheimera and its most closely neighbours were Rheinheimera texasensis A62-14BT and Rheinheimera tangshanensis JA3-B52T with sequence similarities of 98.1 and 97.5%, respectively. The sequence similarities to any other recognized species within Gammaproteobacteria were less than 96.5%. The mean level of DNA-DNA relatedness between strain GR5 T and R. texasensis A62-14BT, the strain most closely related to the isolate, was 26.5 ± 7.6 %. On the basis of the phylogenetic and phenotypic data, strain GR5T should be classified as representing a novel species, for which the name Rheinheimera aquatica sp. nov. is proposed. The type strain is GR5T (= BCRC 80081T = LMG 25379T).
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KEYWORD
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Rheinheimera aquatica sp. nov., antimicrobial activity, polyphasic taxonomy
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